A novel method for culturing human glottic cells.

OBJECTIVES/HYPOTHESIS: The objective of this basic investigation was to describe a new method of preparing primary monolayer cultures of human glottic cells. To our knowledge, this is the first report of the culturing of healthy human glottic cells. This technique may be of use for other applications in the challenging field of laryngeal diseases. STUDY DESIGN: Individual prospective cohort study. METHODS: Tissue samples were collected from 15 patients who underwent laryngeal surgery due to chronic laryngitis or larynx carcinoma. An inverted phase microscope was used to study the cultured cells, and immunocytochemistry using a mouse anti-human cytokeratin 19 monoclonal antibody was performed to identify epithelial cells. The relationship between the culture results and several patient variables was evaluated. RESULTS: Cultures were positive in 40% of samples. The total and supracricoid laryngectomy groups had the highest rate of culture positivity (P < .02). CONCLUSIONS: The current study provides methodological details that will allow other research groups to replicate this model of glottic cell culture.

Desarrollo y validación de un método de análisis de la movilidad ciliar para el diagnóstico precoz de la discinesia ciliar primaria / Development and validation of a method of cilia motility analysis for the early diagnosis of primary ciliary dyskinesia

Introducción: La discinesia ciliar primaria (DCP) es una entidad clínica de difícil diagnóstico debido a que la motilidad y la estructura ciliar pueden variar según los pacientes. El objetivo principal ha sido evaluar la sensibilidad y especificidad para el diagnóstico de la DCP de un sistema de videoanálisis de alta resolución digital junto con un software propio desarrollado por nosotros para el análisis de la movilidad ciliar (Desinsoft-Bio 200). El objetivo secundario ha sido relacionar la actividad nasal ciliar con la clínica y las anomalías estructurales del cilio respiratorio. Material y métodos: Se analizaron el transporte mucociliar mediante un método isotópico, la ultraestructura con microscopia electrónica y la frecuencia y el patrón de batida ciliar mediante el sistema Desinsoft-Bio 200. Veinticinco pacientes con DCP (11 de ellos con síndrome de Kartagener), 27 con discinesia ciliar secundaria y 34 sanos. Resultados: El transporte mucociliar fue defectuoso en los pacientes con DCP y discinesia ciliar secundaria. La inmovilidad ciliar se observó en 6 de los pacientes con síndrome de Kartagener correlacionándose con la ausencia de dineína. Un movimiento ciliar alterado (discinesia) aconteció en el resto de pacientes con DCP, correlacionándose con un déficit parcial de dineína o ulraestructura normal. La movilidad ciliar y la estructura fueron normales en las discinesias ciliares secundarias y en sanos. Discusión y conclusiones: El transporte mucociliar nasal posee una gran sensibilidad cercana al 100% para el diagnóstico de la DCP, pero muy baja especificidad. El sistema de análisis de la movilidad ciliar mediante el sistema de video digital de alta velocidad y resolución tiene una sensibilidad y especificidad elevadas para el diagnóstico de la DCP. Este sistema de videoanálisis es más útil que el estudio ultraestructural y el transporte mucociliar como cribado de la DCP. La ausencia de dineína se correlaciona con la inmovilidad ciliar y es más frecuente en pacientes con síndrome de Kartagener (AU)

Background: Primary ciliary dyskinesia (PCD) is a clinically uniform entity, but cilia motility and structure can vary between patients, making the diagnostic difficult. The aim of this study was to evaluate the sensitivity and specificity in diagnosing PCD of a system of high-resolution digital high-speed video analysis with proprietary software that we developed for analysis of ciliary motility (Desinsoft-Bio 200). The secondary aim was to correlate nasal ciliary activity with clinical and structural abnormalities in PCD. Material and methods: We analysed nasal mucociliary transport, cilia ultrastructure, nasal ciliary beat frequency and beat pattern studied by high-resolution digital high-speed video in 25 cases of PCD (11 Kartagener syndrome), 27 secondary ciliary dyskinesia and 34 healthy volunteers. Results: Nasal mucociliary transport was defective in both primary and secondary ciliary dyskinesia. Ciliary immotility was observed only in 6 patients with Kartagener syndrome and correlated with the absence of dynein. We observed a correlation between partial dynein deficiencies and ciliary dyskinesia. Cilia activity and structure was normal in secondary ciliary dyskinesia. Conclusion: Nasal mucociliary transport showed high sensitivity for PCD diagnosis with a low specificity. High-resolution digital high-speed video has a high sensitivity and specificity for diagnosing PCD. This system of video analysis is more useful than ultrastructural study and mucociliary transport for PCD screening. Dynein absence is correlated with cilia immotility and is more common in patients with Kartagener syndrome(AU)

Development and validation of a method of cilia motility analysis for the early diagnosis of primary ciliary dyskinesia.

BACKGROUND: Primary ciliary dyskinesia (PCD) is a clinically uniform entity, but cilia motility and structure can vary between patients, making the diagnostic difficult. The aim of this study was to evaluate the sensitivity and specificity in diagnosing PCD of a system of high-resolution digital high-speed video analysis with proprietary software that we developed for analysis of ciliary motility (Desinsoft-Bio 200). The secondary aim was to correlate nasal ciliary activity with clinical and structural abnormalities in PCD. MATERIAL AND METHODS: We analysed nasal mucociliary transport, cilia ultrastructure, nasal ciliary beat frequency and beat pattern studied by high-resolution digital high-speed video in 25 cases of PCD (11 Kartagener syndrome), 27 secondary ciliary dyskinesia and 34 healthy volunteers. RESULTS: Nasal mucociliary transport was defective in both primary and secondary ciliary dyskinesia. Ciliary immotility was observed only in 6 patients with Kartagener syndrome and correlated with the absence of dynein. We observed a correlation between partial dynein deficiencies and ciliary dyskinesia. Cilia activity and structure was normal in secondary ciliary dyskinesia. CONCLUSION: Nasal mucociliary transport showed high sensitivity for PCD diagnosis with a low specificity. High-resolution digital high-speed video has a high sensitivity and specificity for diagnosing PCD. This system of video analysis is more useful than ultrastructural study and mucociliary transport for PCD screening. Dynein absence is correlated with cilia immotility and is more common in patients with Kartagener syndrome.

The acromion thoracic flap: A reconstructive resourceafter exeresis of a parotid fibrosarcoma

Sarcomas are rather uncommon in the salivary glands and have a high tendency towards recurrence. The treatmentof choice is surgery, with the first surgical procedure being crucial, as the small size of the tumor is accompaniedby the absence of post-surgery and post-radiotherapy fibrosis, which facilitates the surgery. The evident tendencytowards recurrence makes it advisable to be radical in the exeresis. Often we have to consider a reconstructiveperiod. Negative surgical margins increases the rates of local control. Treatment with radiotherapy is the complementarytreatment indicated for patients with surgical margins less than 2 mm or positive. It has been observedthat this treatment improves the local control of the illness. We present a case of parotid fibrosarcoma of a lowgrade of malignancy that was treated by means of parotidectomy and post-operative radiotherapy. The patient presentedup to three recurrences, one of which required an acromion thoracic flap in order to reconstruct the surgicaldefect. Despite the treatment, the patient ended up dying because one of the recurrences was not operable (AU)

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The acromion thoracic flap: a reconstructive resource after exeresis of a parotid fibrosarcoma.

Sarcomas are rather uncommon in the salivary glands and have a high tendency towards recurrence. The treatment of choice is surgery, with the first surgical procedure being crucial, as the small size of the tumor is accompanied by the absence of post-surgery and post-radiotherapy fibrosis, which facilitates the surgery. The evident tendency towards recurrence makes it advisable to be radical in the exeresis. Often we have to consider a reconstructive period. Negative surgical margins increases the rates of local control. Treatment with radiotherapy is the complementary treatment indicated for patients with surgical margins less than 2 mm or positive. It has been observed that this treatment improves the local control of the illness. We present a case of parotid fibrosarcoma of a low grade of malignancy that was treated by means of parotidectomy and post-operative radiotherapy. The patient presented up to three recurrences, one of which required an acromion thoracic flap in order to reconstruct the surgical defect. Despite the treatment, the patient ended up dying because one of the recurrences was not operable.